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    2
    Growing cultures of AMJ2-C11 were collected and cell debris was removed by centrifugation (3000 g, r
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    otor GH-3.8, Beckman) for 15 min. After clarification, the supernatants were filtered through a 0.45-m membrane (Millipore, Bedford, MA), diluted 1/1 with DMEM conditioned Medium containing 6 g/ml of polybrene (final concentration), and added to the primary cultures for overnight at 37 .Immortalizat
    2
    Growing cultures of AMJ2-C11 were collected and cell debris was removed by centrifugation (3000 g, r
    Read More
    otor GH-3.8, Beckman) for 15 min. After clarification, the supernatants were filtered through a 0.45-m membrane (Millipore, Bedford, MA), diluted 1/1 with DMEM conditioned Medium containing 6 g/ml of polybrene (final concentration), and added to the primary cultures for overnight at 37 .Immortalizat
    1
    Breast tumors, colorectal cancer cells often show an overlapping growth pattern in the examination s
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    pecimen making FISH analysis less reproducible and harder to interpret. The CISH technique seems able to overcame this potentially confounding factor as it offers the opportunity to morphologically identify overlapping tumor cells, which can be consequently excluded from the analysis. In our series
    1
    Breast tumors, colorectal cancer cells often show an overlapping growth pattern in the examination s
    Read More
    pecimen making FISH analysis less reproducible and harder to interpret. The CISH technique seems able to overcame this potentially confounding factor as it offers the opportunity to morphologically identify overlapping tumor cells, which can be consequently excluded from the analysis. In our series
    1
    Breast tumors, colorectal cancer cells often show an overlapping growth pattern in the examination s
    Read More
    pecimen making FISH analysis less reproducible and harder to interpret. The CISH technique seems able to overcame this potentially confounding factor as it offers the opportunity to morphologically identify overlapping tumor cells, which can be consequently excluded from the analysis. In our series
    1
    Ure Collection (ATCC, Manassas, VA); RPMI 1640 medium and PBS were purchased from BioWhittaker (Walk
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    ersville, MD); Gentamycin, penicillin/streptomycin, macrophage-colony stimulating factor (M-CSF), and Polybrene were obtained from Sigma (St. Louis, MO); MethoCultTM GF M3434, semi-solid medium was obtained from StemCell Technologies (Vancouver, Canada); DiffQuik, a modified Wright-Giemsa stain was
    1
    Ure Collection (ATCC, Manassas, VA); RPMI 1640 medium and PBS were purchased from BioWhittaker (Walk
    Read More
    ersville, MD); Gentamycin, penicillin/streptomycin, macrophage-colony stimulating factor (M-CSF), and Polybrene were obtained from Sigma (St. Louis, MO); MethoCultTM GF M3434, semi-solid medium was obtained from StemCell Technologies (Vancouver, Canada); DiffQuik, a modified Wright-Giemsa stain was
    1
    Ure Collection (ATCC, Manassas, VA); RPMI 1640 medium and PBS were purchased from BioWhittaker (Walk
    Read More
    ersville, MD); Gentamycin, penicillin/streptomycin, macrophage-colony stimulating factor (M-CSF), and Polybrene were obtained from Sigma (St. Louis, MO); MethoCultTM GF M3434, semi-solid medium was obtained from StemCell Technologies (Vancouver, Canada); DiffQuik, a modified Wright-Giemsa stain was
    1
    Bs: pre-incubate the cells with 0.5 g of mAb 2.4G2 (anti-mouse CD16/CD32, to block binding of FcR) p
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    er million cells for 5?0 minutes on ice prior to staining, then add 0.25 g/ml of FITC-labeled anti-mouse F4/80 or FITC-labeled anti-mouse CD11b to the cells in each tube, mix gently and incubate for 30 min in the dark. All staining procedures were carried out at 4 . Cells were then washed 3 times an
    1
    Pacity of latex beads similar to primary AMs deficient in MARCO and SR-AI/II. All three clones showe
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    d significantly decreased uptake of fluorescent latex beads compared to the wild type primary AMs (Fig. 6A). Observed differences in phagocytic capacity between these clones and parental primary AMs from MS-/- mice may reflect the heterogeneity seen in populations of primary alveolar macrophages.ZK1