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    In at 14,000 g; remove upper phase (without disturbing the interphase, which contains the proteins); add 450 MeOH; vortex thoroughly; spin 2 min; remove supernatant; air dry pellet 15 min. Sample preparation To protect against protease activity, we added a mix of protease inhibitors (inhibitor mix) to solutions before the 1st dimension: 1 mM EDTA, 10 E64c, 0.002 mg/ml Aprotinin and 1 Pepstat
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